척수 아교질 세포의 흥분성과 세포내 칼슘농도에 대한 활성산소의 영향에 대한 연구
Changes in neuronal excitability and intracellular calcium concentration by reactive oxygen species on spinal substantia gelatinosa neurons
이해인, 천상우,
소속 상세정보
이해인 ( Lee Hae In ) - 원광대학교 치과대학 생리학교실 및 원광 생채재료매식 연구소
천상우 ( Chun Sang-Woo ) - 원광대학교 치과대학 생리학교실 및 원광 생채재료매식 연구소
KMID : 0353320110350010015
Abstract
Substantia gelatinosa (SG) neurons, lamina II of the spinal dorsal horn, receive sensory informations from peripheral neurons directly and send the informations to upper brain regions. In recent studies, it is reported that reactive oxygen species (ROS) takes part in generation and transmission of pain, but these are the results from behavioral test and immunohistochemical study. In the present study, we recorded neuronal excitability to investigate the effects of tert-buthyl hydroperoxide (t-BuOOH), a kind of ROS, on transmission of pain in SG using the patch clamp technique. We also used calcium imaging technique to measure the changes of intracellular calcium concentration. Depolarization and action potential were evoked by perfusion of t-BuOOH, and these are suppressed by phenyl-N-tert-buthylnitrone, an antioxidant. When we treated t-BuOOH, intracellular calcium concentration was increased, but it was rather decreased in extracellular calcium free solution and on the condition of pretreated 2-aminoethoxydiphenylbolate, ruthenium red, and thapsigargin. We confirmed that t-BuOOH activates excitability of neurons in SG suggesting that ROS boosts nociception through central sensitization. This result is induced by increase of intracellular calcium, and the sources of calcium are from extracellular fluid and endoplasmic reticulum in neurons.
키워드
Endoplasmic reticulum; Intracellular calcium concentration; ROS; SG neuron
원문 및 링크아웃 정보
등재저널 정보